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Objective To evaluate the randomness and representativeness of respondent-driven sampling (RDS) tool in conducting the investigation in MSM population,in Beijing,2017.Methods RDS tool was used to recruit MSM population for a face-to-face interview with structured questionnaire and serological tests.Results A total of 600 MSM people were sampled and interviewed.The median number of personal network of seeds was 10,which was higher than other MSM people recruited.The numbers of recruitments by wave presented a skewed positive distribution and the highest number was in the fourth wave.It was also dramatically varied from different seeds.Three seeds had the longest chains and had recruited 184,113 and 92 MSM people,respectively.In contrast,five seeds recruited less than 10 MSM people.Two college students were the most non-generative seeds and each recruited only 1 MSM person.After five to nine waves of sampling,the major demographic characteristics reached equilibrium.Both convergence and bottleneck plots of major demographic characteristics reached convergence,although the plots on marriage and education did not.The homophiles of characteristics were all closed to 1,except for education.The HIV positive rate appeared as 7.9％ (95％CI:4.4％-11.4％). Conclusions Results from this study showed that RDS could be used as a feasible sampling method for the study on MSM population with major demographic characteristics reached equilibrium.The process of recruitment appeared controllable and reasonable,showing that this could represent the MSM population in Beijing,in some degree.
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Objective To evaluate the randomness and representativeness of respondent-driven sampling (RDS) tool in conducting the investigation in MSM population,in Beijing,2017.Methods RDS tool was used to recruit MSM population for a face-to-face interview with structured questionnaire and serological tests.Results A total of 600 MSM people were sampled and interviewed.The median number of personal network of seeds was 10,which was higher than other MSM people recruited.The numbers of recruitments by wave presented a skewed positive distribution and the highest number was in the fourth wave.It was also dramatically varied from different seeds.Three seeds had the longest chains and had recruited 184,113 and 92 MSM people,respectively.In contrast,five seeds recruited less than 10 MSM people.Two college students were the most non-generative seeds and each recruited only 1 MSM person.After five to nine waves of sampling,the major demographic characteristics reached equilibrium.Both convergence and bottleneck plots of major demographic characteristics reached convergence,although the plots on marriage and education did not.The homophiles of characteristics were all closed to 1,except for education.The HIV positive rate appeared as 7.9％ (95％CI:4.4％-11.4％). Conclusions Results from this study showed that RDS could be used as a feasible sampling method for the study on MSM population with major demographic characteristics reached equilibrium.The process of recruitment appeared controllable and reasonable,showing that this could represent the MSM population in Beijing,in some degree.
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Objective To discuss the impact of different markers on the estimation of MSM using the capture-recapture method.Methods From March to July 2013,MSM were captured from the their related network to estimate the site of this population,under the capture-recapture method.Two different markers were set in the process of first ‘capture'.Results The estimated numbers of MSM in Beijing were 239 258 (95％CI:48 932-429 584) and 115 731 (95％CI:78 153-153 309) when using the two markers under capture-recapture method.Conclusion The estimated size of MSM population varied greatly under different markers which called for the right selection on markers when using the capture-recapture method to estimate the size of specific population.
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Objective To determine the prevalence of and correlations between HIV infection and syphilis among men who have sex with men (MSM) in Beijing.Methods Totally,600 MSM collected from sentinel surveillance sites for AIDS in Beijing were recruited in this study,and underwent screening tests for HIV infection and syphilis.Enzyme-linked immunosorbent assay (ELISA) was used for the primary screening for HIV infection,and Western blot for its confirmation.Both nontreponemal test (rapid plasma regain (RPR) assay) and treponemal test (Treponema pallidum particle agglutination assay,TPPA) were performed to detect syphilis,and only those with a reactive TPPA result were defined as having syphilis (including past infection).Specimens confirmed as HIV-1 positive were further tested with BED-capture enzyme immunoassay (CEIA) to find recent HIV-1 infection,and the incidence of HIV infection was then calculated.The correlation between syphilis and HIV infection was statistically analyzed.Results Of the 600 MSM,49 (8.17％) had HIV infection,including 14 cases of recent infection,with the incidence rate of HIV infection being 4.68％; 51 were positive for both RPR and TPPA tests with the infection rate (including past infection) being 18.5％ (111/600).People without a Beijing household registration and those with high education levels accounted for a large proportion of HIV-infected MSM.Conclusions The prevalence and incidence of HIV infection as well as prevalence of syphilis maintain at a relatively high level in MSM in Beijing.HIV infection is markedly correlated with syphilis in MSM population.
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Objective:To observe the effect of "Qishen Yiqi Droplet" on serum concentrations of high sensitivity C reactive protein(hs-CRP),plasminogen activator inhibitor-1(PAI-1),endothelin-1(ET-1)in patients with acute coronary syndrome(ACS)after percutaneous coronary intervention (PCI).Methods:A total of 100 consecutive ACS patients ready to receive PCI therapy in our hospital from june 2007 to August 2008 were randomly assigned into two groups:Qishen group,n=50,with Qishen Yiqi Droplet 5.0 g three timee a day+conventional therapy after PCI;and Control group,n=50 with conventional therapy after PCI.The concentrations of hs-CRP,PAI-1 and ET-1were detected and compared 24 hours before PCI,and 24 hours,4weeks after PCI between two groups respectively.Results:There were no obvious differences of hs-CRP,PAI-1 and ET-1 24 hours after PCI in two groups.The levels of hs-CRP,PAI-1,ET-1 were lower in Qishen group than in Control group 4 weeks after PCI (0.219±0.143)ng/dl vs.(0.366±0.132)ng/dl,P<0.001,(104.252±26.038)ng/dl vs.(118.419±28.849)ng/ml,P<0.05,and (37.411±12.977)ng/dl vs.(45.755±12.305)pg/ml,P<0.01,respectively.Conclusion:Qishen Yiqi Droplet could decrease the levels of hs-CRP,PAI-1 and ET-1 in ACS patients who underwent PCI,and it might have the protective role in preventing the neointimal hyperplasia and thrombogenesis after stent implantation.
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Zishen Tiaochong therapy can improve the function of corpus luteum.The function of corpus luteum has close correlation with vasculogenesis in which the bone marrow-derived endothelial progenitor cells(EPCs)were involved.This implies the Zishen Tiaochong therapy may be correlative to vasculogenesis in corpus luteum via mobilizing bone marrow-derived EPCs.In this paper,based on the TCM theory of 'kidney(Shen) storing essence,producing bone marrow,controlling reproduction',we review the topic of Zishen Tiaochong therapy perfects the function of corpus luteum combined the new mechanisms of research advances in vasculogenesis.The possible mechanisms of promoting vasculogenesis in corpus luteum by Zishen Tiaochong therapy were discussed and analyzed.